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If it is suspected that the expressed protein is toxic to the cells (usually they will stop growing after induction with IPTG), try inducing at a later time point for a shorter amount of time

These findings were corroborated in the work presented by Song et al., who showed that ovarian cancer could induce endoplasmic reticulum stress in cytotoxic T cells, which depleted glutamine transporters in these cells and subsequently led to T-cell dysfunction [152]

While glutamate and aspartate can overexcite neurons, taurine may act as a compensatory calming agent

Thomas R, Al-Khadairi G, Roelands J et al (2018) NY-ESO-1 based immunotherapy of cancer: current perspectives

S.BrietzkeE.RosenblatJ

This guide covers the immunology, the specific peptides under investigation, the dosing protocols documented in research, and the practical considerations anyone exploring this area needs to know