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Zemba Cilic et al., 2021)

The benzyl alcohol preservative inhibits bacterial growth Sterile water has a shorter window If reconstituted with plain sterile water (no preservative), use within 48 hours or freeze in single-use aliquots Avoid freeze-thaw cycles Repeatedly freezing and thawing a reconstituted peptide solution causes degradation

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Corresponding author: Karin Elgar (email [email protected])

The DMMB assay 35 provided precise measures of GAG release which complemented the semi-quantitative grading of GAG loss used in the cross-sectional comparison of herniated and degenerated discs