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Upregulation of genes associated with the expression of glutathione (GSH), glutathione transferase (GST), and cytochrome P450 oxidases (CYP450) has been observed in potential loser cells, with most of these genes being targets of Nrf2 (231)

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Viability assay In 96-well plates, cells were seeded at 5.0 10 3 per well

Glutathione is called an anti-oxidant because it traps free radicals, transports them to the liver and then goes back to work regenerating itself

10.1016/j.redox.2013.01.007 Redox Biol

For seniors: Youre more likely to have problems with their liver, kidneys, or bone marrow while taking methotrexate