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Phosphorus was lowest in DOGROF-3 (0.97%) and highest in DOGROF-4 (2.37%), while potassium ranged from 3.42% in DOGROF-3 to 23.59% in DOGROF-4

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doi: 10.2174/187152011795677490

After reaching confluency (7080%), cells were cultured with calcifying medium with or without DT-109 for 5 days for Western blot analysis or 10 days for subsequent staining and calcium content determination