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Nitric oxide activates superoxide dismutase and ascorbate peroxidase to repress the cell death induced by wounding

The polyphenols found in tea, especially green tea, can reduce digestion and absorption of carbohydrates, lipids and proteins and can affect gut microbiota and possibly the gutbrainliver axis (Reference Yang, Zhang and Zhang51)

We modified and optimized our previous synthetic procedure to exclusively obtain GGDNBS

However, since cell counting is only a rough proxy to assess cytotoxicity and a higher dose escalation is necessary for CC 50 determination, we further included careful assessment of growth kinetics by live cell imaging, measurement of mitochondrial metabolic activity by MTT assay as well as cellular ATP levels by CellTiter-Glo (CTG, see Material and Methods section for details)

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The non-inoculated YPD broths (pH 2 and pH 3) were calibrated as the control blanks respectively and the growth index was calculated as shown in equation [1]