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Reactive astrocytes contribute to pain hypersensitivity through activation of the JAKSTAT3 pathway 266 with consequent release of cytokines, including tumor necrosis factor (TNF), interleukin (IL)-1 (IL-1) and CC motif chemokine 2 (CCL2) 267,268,269,270

Serum was isolated from the blood by centrifuging blood samples at 10,000 g for 5 min and stored at 80 C

At 3.5 hours (Fig

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