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Treated mice showed decreased susceptibility to muscle damage, improved muscle strength, reduced PARP activity, increased NAD + levels, and elevated mitochondrial function 254 , thus suggesting a potential therapeutic benefit of NR supplementation in DMD

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1 mL of 80 C 80% MeOH was added to the wells, samples were incubated at 80 C for 20 min, then cells were scraped off and supernatants were transferred into microcentrifuge tubes