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The connective tissue and the gall bladder were removed, and the liver was minced into small pieces and forced gently through a 200-mm-gauge stainless steel mesh using a sterile syringe plunger and then suspended in 50 ml Roswell Park Memorial Institute (RPMI)-1640 medium containing GlutaMAX-1, 25 mM HEPES and 10% FCS (pH 7.4)

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MOTS-c can be added for metabolic optimization and mitochondrial support

10.1093/annonc/mdy001 52 RemonJ.SoriaJ.-C.PetersS.ESMO Guidelines Committee

Explants were incubated overnight at 4C with mouse monoclonal antibody against III-tubulin (Millipore, MAB5564) diluted 1:10,000 in blocking solution

doi: 10.1016/s0022-510x(97)00146-9