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Again, this impairment is effectively bypassed by supplementation with gamma-glutamylcysteine, which can be transported into cells where it will be rapidly converted to GSH by GSH synthase

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To exclude false negatives, all investigated GSTs were confirmed to be solubly and functionally produced by SDSPAGE analysis and an activity test with the generic GST substrate CDNB ( 5 ) (Extended Data Fig

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As an alternative to HPLC, ion exchange chromatography also could be performed