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Description
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The reaction was quenched by transferring 50 L of the sample to 200 L of internal standard solution, followed by homogenization (Eppendorf Mixmate, 1200 rpm, 5 min) and centrifugation (4000 rpm, 15 min, 4C)
In patients with cirrhosis , portal hypertension, and large portosystemic shunts, ammonia from intestinal sources is not cleared effectively on first-pass through the liver
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