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LysoTracker red DND99 (Introgen, Eugene, Oregon, USA), an acidotropic dye and a weak base conjugated to a red fluorophore, was added to the cells on the designated coverglass wells on the day of infection

Though FAD did not directly relate to histamine on SEM analysis, insufficient FAD for good MTHFR activity may provide one explanation since undermethylating environments with insufficient SAMe available for the histamine-metabolizing enzyme N methyl histamine means that there is a tendency for histamine to be conserved and elevated (60)

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Translational Control Protein 80 Stimulates IRES-Mediated Translation of p53 mRNA in Response to DNA Damage

J Physiol Biochem

doi: 10.1007/s00702-014-1180-8