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Cell pellet was resuspended in cell lysis buffer (50 mM TrisHCl, 150 mM NaCl, 1% TritonX-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM EDTA and 50 mM sodium fluoride) containing 1 mM DTT, 1 mM PMSF and protease inhibitor cocktail