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Fresh leaf sample grounded in extraction buffer comprising of Na-phosphate (0.5 M, pH 7.3), EDTA (3 mM), Triton X 100 (1% v/v) and PVP (1% w/v)

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When iron is released from ferritin, it can form an Fe(2+)/Fe(3+) complex and start the chain reaction of lipid oxidation [35]

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