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Carnitine, Acylcarnitines and Fatty Acid Transport into Mitochondria The substrates for oxidation and most of the energy production in cells are unesterified fatty acids, which originate from three main sources: exogenous fatty acids that enter cells from the circulation, either from the diet or by mobilization from other tissues, those that arise via synthesis de novo from acetyl-CoA, and those released within the cell by the hydrolysis of phospholipids and triacylglycerols

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Glycine propionyl-l-carnitine is created through binding of the amino acids glycine and propionyl-L-carnitine (PLC)

Fluorescent cross-sectioned images of the soleus were segmented by myosin fiber types (slow MyHC or MyHC IIA) using a pixel classifier developed in ilastik

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Visualization was performed using the OLYMPUS CellSens v2.3 Imaging software