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A blocking buffer [10% BSA in PBS-T Buffer (0.05% Tween-20 v/v)] was added for 1 h at room temperature to minimize non-specific antibody binding

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Tanshinone IIA is a fat-soluble active ingredient of salvia miltiorrhiza, with anticoagulant, anti-inflammatory, antioxidant, anti-fibrotic, and immunomodulatory effects (75)

For patients taking tirzepatide, which enhances insulin secretion in a glucose-dependent manner, this risk requires careful consideration