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The final concentration was adjusted to 22% using Pluronic F127

Next, the slides were stained with immunoperoxidase using a General SP kit (SP-9000, ZSGB-BIO, Beijing, China), developed with 3,3-diaminobenzidine (ZLI-9019, ZSGB-BIO), and counterstained with hematoxylin

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L-carnitine L-tartrate by HSN is presented in powder form, with a small particle diameter

Statistical analysis For animal studies, sample sizes ( n = 5 per group, 3 in the tirzepatide-treated group) were determined based on prior in-house experience and previous studies using similar mouse models to ensure adequate power to detect biologically relevant effects

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