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The animals were anesthetized by immersion in a solution of tricaine (300 mg/L, CAS number 886-86-2) until loss of motor coordination and reduction of respiratory rate

[DOI] [PubMed] [Google Scholar] 152.Llull L, Laredo C, Ren A, et al

Through this coordinated regulation of both adaptive and innate immune components, SFN establishes a more immunostimulatory microenvironment that not only enhances direct tumor cell killing but also creates favorable conditions for combination strategies with various immunotherapeutic approaches

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[DOI] [PubMed] [Google Scholar] 207.Uchimura T

Ascorbic Acid is also needed for: Carnitine synthesis Tyrosine synthesis and catabolism Serotonin (neurotransmitter) synthesis Other hormone and neurotransmitter synthesis6 The figure below shows how ascorbic acid is needed for dopamine hydroxylase, which ultimately produces the hormone epinephrine