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Reduced release of fatty acids and glycerol from adipose tissue directly links adipose tissue metabolism to that of the liver

Both uniformly C13-labeled palmitic acid (UC13-C16:0, Larodan) and docosahexaenoic acid (DHA, Cayman Chemicals) were conjugated with albumin (using Fatty Acid Free Bovine Serum Albumin, BSA, Goldbio) to a final concentration of 2 mM and 40 M, respectively, and aliquoted out into 5 ml glass scintillation vials stored at 20C

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Additional limitations include substantial variability in polyphenol composition across foods, a lack of well-designed human intervention studies specifically targeting legume-derived polyphenols, and pronounced interindividual differences in microbiota composition that affect metabolic responses

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