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Merck), 200 g/mL RNase A (Nacalai Tesque, Kyoto, Japan) in 0.2% PBST] at 37 C for 15 min

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Furthermore, regulatory T (Treg) cells were upregulated and Th17 cells were downregulated in genistein-treated NOD/LtJ mice compared with untreated NOD/LtJ mice (Supplementary Fig

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5254 These approaches use large variant libraries and high-throughput selection to map sequencefunction relationships, which is particularly useful for protein engineering and understanding fitness landscapes