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Immunofluorescence in primary cortical neurons Micro Coverglass #1.5 coverslips (Electron Microscopy Sciences) coverslips were washed in 2 M HCl overnight at 55 C, washed 5 times in sterile H 2 O, then pre-coated with poly-D-lysine (50 g/mL) overnight at 37 C, then washed with distilled water three times and air-dried at room temperature for at least 2 h

Today, DSIP is primarily studied as a peptide of interest in neuropeptide pharmacology, signaling pathway investigation, and molecular regulatory network research

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Nucleosides isolated from Ophiocordyceps sinensis inhibit cigarette smoke extract-induced inflammation via the SIRT1-nuclear factor-B/p65 pathway in RAW264.7 macrophages and in COPD mice