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Research shows clear benefits: Better stability than oral delivery, which exposes peptides to harsh stomach conditions Less breakdown by enzymes that would destroy the peptide before it reaches the blood Easier compliance no needles, no mixing, just spray Potential for targeted delivery to specific tissues, including the brain via the olfactory pathway Nasal delivery works very well for peptides like BPC-157 that target the whole body

For GSH feeding, freshly prepared 100 M GSH was used while for BSO treatment, 1 mM BSO was used for 72 h as standardized before (Datta et al., 2015)

2019 May 27;3(2):200-214

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The prepared supernatants reacted with Assay Buffer and Iron Reducer for 30 min