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Coronal slices (300 m) were prepared using a vibrating microtome (PRO7N

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Alemany S, Avella-Garca C, Liew Z, Garca-Esteban R, Inoue K, Cadman T, Lpez-Vicente M, Gonzlez L, Riao Galn I, Andiarena A, Casas M, Margetaki K, Strandberg-Larsen K, Lawlor DA, El Marroun H, Tiemeier H, Iiguez C, Tardn A, Santa-Marina L, Jlvez J, Porta D, Chatzi L, Sunyer J

After 23 days, the expansion medium was replenished without Y-27632, and the medium was refreshed every 23 days thereafter until the next passage