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A pure RBC suspension can reliably be achieved by density-gradient centrifugation (2500 RPM, 15 min, RT)

Immunologic and endocrine effects of the flame-retardant pentabromodiphenyl ether (DE-71) in C57BL/6 J mice

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The animal research, while promising, doesn't translate directly to human use, and the FDA has determined that insufficient safety data exists for compounding

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The documented research protocol is once-daily dosing in the activation phase (roughly weeks 1-4), then the same per-dose amount at a reduced 3-5x/week frequency in maintenance