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After 10 min, the clotted blood samples were centrifuged for 5 min at 3000 rpm , and then serum was separated and transported into three new Eppendorf tubes until assay

A multifunctional serine protease primes the malaria parasite for red blood cell invasion

doi: 10.1104/pp.126.3.1012 147

Three to four microliters of VLP-compound or VLP-compound-GSH mixture were applied to glow-discharged Lacey carbon copper grids with an ultra-thin carbon support film (product No