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The appearance of the vessels was assessed (see also Figure 1) between (1) the last arcade proximal to first ligation (arcade 0) and the first arcade distal to the ligation (or to the previous ligation) (arcade 1) (0-1, Figure 2, Figures 5-8, and Figures 21 and 22)

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Some protocols use an extended break matching the cycle length (8 weeks on, 8 weeks off)