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(2019) and calculated as: Enzyme assays For the enzyme assays, the supernatant of a centrifuged (10,000 g for 20 min, at 4 C) composite sample of mango pulp (from 8 fruit from each replication) at eating soft ripe stage previously homogenised in a potassium citrate buffer (cold, pH 4.0, 4 mL) was used

Elevated homocysteine levels suggests an enzymatic block early in the pathway of methionine metabolism

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The high GH levels, together with the often severe IGF-1 deficiency, will lead to a severe catabolic state, including significant lipolysis and proteolysis, comparable to extended fasting during a hunger strike, for instance [26]

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