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Description
Cognitive function was evaluated with the Morris water maze and CNS cytokine expression was measured by multiplex assay

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Following a final saline wash, 50% glycerol was added to the wells, and bright-field images were taken using an inverted microscope

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The peripheral blood shows evidence of hemolysis and macrocytosis
Slides were cut for each biopsy in 5 m sections, and stained with hematoxylin and eosin to confirm menstrual cycle phase using the Noyes criteria 43
