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At the end of the experiment, the whole fish in each tank were anthesthetized on ice, and sampled to collect liver, muscle and visceral for molecular and biochemical indexes

[DOI] [PMC free article] [PubMed] [Google Scholar] 92.Osman D., Piergentili C., Chen J., Chakrabarti B., Foster A.W., Lurie-Luke E., Huggins T.G., Robinson N.J

For the MS/MS scan the following conditions were used: NCE at 20, 30, and 40 a.u

The concentrated methanol solution containing the targeted fragment underwent further purification via repeated injection into an HPLC-UV system (Agilent Technologies 1100 Series HPLC) equipped with a diode-array detector, using a Nucleodur Sphinx RP column (250 4.6 mm 5 m)

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Getting the site wrong going too deep, hitting the same spot repeatedly, or skipping basic sterility is the most avoidable source of bruising, lumps, and irritation