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Ophardt CE

A key randomized, double-blind, placebo-controlled crossover trial revealed that NR supplementation (1000 mg daily) for 6 weeks increased blood cellular NAD+ concentrations by approximately 60% compared to placebo [4]

L-Glutathione is commonly used in controlled in-vitro experiments modeling oxidative stress and antioxidant pathway behavior

Shown below is the structure of glutathione with the pka values of its acidic residues indicated

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Sections were imaged using an optical microscope (Nikon Corporation) with a 400 objective, and the cells with positive staining were quantified using ImageJ 2.0.0 (National Institutes of Health)