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Laboratory investigation

10.1074/jbc.M211558200 J

Gao Y, Li H, Song Y, Zhang F, Yang Z, Yang Y, Grohmann T (2022) Response of glutathione pools to cadmium stress and the strategy to translocate cadmium from roots to leaves ( Daucus Carota L)

One of the most commonly used fusion tags for recombinant protein expression and purification is the His tag

In brief, RNA was extracted from cells of the indicated genotype using the Qiagen RNAeasy RNA extraction kit (Qiagen, Manchester, UK) following the manufacturers instructions.mRNA (1 g) was retrotranscribed into cDNA using High Capacity RNA-to-cDNA kit (Life Technologies, Paisley, UK)

and Mieyal J.J