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After being washed with phosphate buffered solution (PBS) and fixed in 4% prewarmed (37 C) paraformaldehyde for 15 min, the cells were permeabilized with 0.5% Triton X-100 for 15 min and blocked for 30 min in 10% goatserum

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The epithalamin-treated group also had reduced rates of coronary artery disease, hypertension, and osteoarthritis, compared to the control group participants [6]

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Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery